期刊
PLOS ONE
卷 9, 期 1, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0086295
关键词
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资金
- National High Technology Research and Development Program 863 [2012AA02A508]
- China National Natural Scientific Fund [81072078, 81172389, 81101901]
- Jiangsu Province's Natural Science Foundation [BK2010580, 2011847]
- Jiangsu Province's Key Discipline of Medicine [XK201117]
- Jiangsu Province's Medical Major Talent program [RC2011051]
- Program for Development of Innovative Research Team in the First Affiliated Hospital of NJMU
- Provincial Initiative Program for Excellency Disciplines, Jiangsu Province
H19 RNA has been characterized as an oncogenic long non-coding RNA (lncRNA) in breast and colon cancer. However, the role and function of lncRNA H19 in glioma development remain unclear. In this study, we identified that H19/miR-675 signaling was critical for glioma progression. By analyzing glioma gene expression data sets, we found increased H19 in high grade gliomas. H19 depletion via siRNA inhibited invasion in glioma cells. Further, we found H19 positively correlated with its derivate miR-675 expression and reduction of H19 inhibited miR-675 expression. Bioinformatics and luciferase reporter assays showed that miR-675 modulated Cadherin 13 expression by directly targeting the binding site within the 3' UTR. Finally, introduction of miR-675 abrogated H19 knockdown-induced cell invasion inhibition in glioma cells. To our knowledge, it is first time to demonstrate that H19 regulates glioma development by deriving miR-675 and provide important clues for understanding the key roles of lncRNA-miRNA functional network in glioma.
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