OptZyme: Computational Enzyme Redesign Using Transition State Analogues

OptZyme is a new computational procedure for designing improved enzymatic activity (i.e., k(cat) or k(cat)/K-M) with a novel substrate. The key concept is to use transition state analogue compounds, which are known for many reactions, as proxies for the typically unknown transition state structures. Mutations that minimize the interaction energy of the enzyme with its transition state analogue, rather than with its substrate, are identified that lower the transition state formation energy barrier. Using Escherichia coli beta-glucuronidase as a benchmark system, we confirm that K-M correlates (R-2 = 0.960) with the computed interaction energy between the enzyme and the para-nitrophenyl- beta, D-glucuronide substrate, k(cat)/K-M correlates (R-2 = 0.864) with the interaction energy of the transition state analogue, 1,5-glucarolactone, and k(cat) correlates (R-2 = 0.854) with a weighted combination of interaction energies with the substrate and transition state analogue. OptZyme is subsequently used to identify mutants with improved K-M, k(cat), and k(cat)/K-M for a new substrate, para-nitrophenyl-beta, D-galactoside. Differences between the three libraries reveal structural differences that underpin improving K-M, k(cat), or k(cat)/K-M. Mutants predicted to enhance the activity for para-nitrophenyl- beta, D-galactoside directly or indirectly create hydrogen bonds with the altered sugar ring conformation or its substituents, namely H162S, L361G, W549R, and N550S.