期刊
ZYGOTE
卷 24, 期 4, 页码 537-548出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0967199415000507
关键词
Apoptosis genes; Blastocyst; GSH level; Goat oocyte; alpha-Linolenic acid
资金
- Stem Cell Technology Research Center, Tehran, Iran
The effects of alpha-linolenic acid (ALA) on developmental competence of oocytes in goats were evaluated in this study. Initially, the level of ALA in small and large antral follicles was determined to be in a range of 0.018-0.028 mg/ml (64.6-100.6 mu M, respectively). In vitro maturation was performed in the presence of various concentrations (10, 50, 100, or 200 mu M) of ALA. Cumulus expansion, meiotic maturation, levels of intracellular glutathione (GSH), embryonic cleavage, blastocyst formation following parthenogenetic activation (PA) and in vitro fertilization (IVF), number of total and apoptotic cells in blastocyst, and expression of Bax, Bcl-2, and p53 genes in blastocyst cells were determined. Compared with the control, no improvement was observed in cumulus expansion in ALA-treated groups. At 50 mu M concentration, ALA increased meiotic maturation rate but had no effect on GSH level. When oocytes treated with 50 mu M ALA were subsequently used for PA or IVF, a higher rate of blastocyst formation was observed, and these embryos had a higher total cell number and a lower apoptotic cell number. Expression analyses of genes in blastocysts revealed lesser transcript abundances for Bax gene, and higher transcript abundances for Bcl-2 gene in 50 mu M ALA group. Expression of p53 gene was also less observed in ALA-treated blastocysts. Our results show that ALA treatment at 50 mu M during in vitro maturation (IVM) had a beneficial effect on maturation of goat oocytes and this, in turn, stimulated embryonic development and regulated apoptotic gene expression.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据