期刊
PLOS ONE
卷 8, 期 11, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0080434
关键词
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资金
- National Basic Research Program of China (973 Program) [2012CB721102]
- National Natural Science Foundation of China [30830002]
- China Postdoctoral Science Foundation funded project [2012T50444, 2012M520947]
Streptomyces phage phi BT1 integrates its genome into the attB site of the host chromosome with the attP site to generate attL and attR. The phi BT1 integrase belongs to the large serine recombinase subfamily which directly binds to target sites to initiate double strand breakage and exchange. A recombination directionality factor (RDF) is commonly required for switching integration to excision. Here we report the characterization of the RDF protein for phi BT1 recombination. The RDF, is a phage-encoded gp3 gene product (28 KDa), which allows efficient active excision between attL and attR, and inhibits integration between attB and attP; Gp3 can also catalyze topological relaxation with the integrase of supercoiled plasmids containing a single excision site. Further study showed that Gp3 could form a dimer and interact with the integrase whether it bound to the substrate or not. The synapse formation of attL or attR alone with integrase and Gp3 showed that synapsis did not discriminate between the two sites, indicating that complementarity of central dinucleotides is the sole determinant of outcome in correct excision synapses. Furthermore, both in vitro and in vivo evidence support that the RDFs of phi BT1 and phi C31 were fully exchangeable, despite the low amino acid sequence identity of the two integrases.
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