Simultaneous Identification and Quantification of 20 β-Receptor Agonists in Feed Using Gas Chromatography-Tandem Mass Spectrometry

Lean meat powder is a class of toxic chemicals that have structures similar to that of beta-adrenergic receptor agonists. At least 16 chemicals from this class have been specifically banned by the 176th bulletin of the Chinese Department of Agriculture on breeding animals, and methods for monitoring the illicit use of beta-agonists in animal feed are required. Herein, a method to quantify 20 beta-agonists in feed, via analyte derivatization followed by gas chromatography-tandem mass spectrometry, has been developed. The optimized method has a good linear correlation (calibration coefficient > 0.99) between the quantitative ion peak area and the concentration of beta-agonists over a large working range (0.05-1 mg/kg). The limit of detection (LOD) was 0.01 mg/kg, the recoveries for three beta-agonists spikes (0.05, 0.1, and 1 mu g/g) in feed ranged from 75.6 to 102.4%, repeatability ranged from 1.2 to 9.4% for all of the compounds, and intermediate precisions were lower than 13.8%. This precise, accurate method was applied to quantify 20 beta-agonists in actual feed samples and represents an excellent complement to existing quantification methods.