期刊
PLOS ONE
卷 8, 期 9, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0074678
关键词
-
资金
- Ministry of Agriculture of China [2008ZX08002-001-07, 2011ZX08002-001-07]
Heavy nitrogen (N) application to gain higher yield of wheat (Triticum aestivum L.) resulted in increased production cost and environment pollution. How to diminish the N supply without losing yield and/or quality remains a challenge. To meet the challenge, we integrated and expressed a tobacco nitrate reductase gene (NR) in transgenic wheat. The 35S-NR gene was transferred into two winter cultivars, Nongda146 and Jimai6358, by Agrobacterium-mediation. Over-expression of the transgene remarkably enhanced T-1 foliar NR activity and significantly augmented T-2 seed protein content and 1000-grain weight in 63.8% and 68.1% of T-1 offspring (total 67 individuals analyzed), respectively. Our results suggest that constitutive expression of foreign nitrate reductase gene(s) in wheat might improve nitrogen use efficiency and thus make it possible to increase seed protein content and weight without augmenting N supplying.
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