期刊
PLOS ONE
卷 8, 期 5, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0064462
关键词
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资金
- Swedish Research Council
- NovoNordisk Foundation
- Region Skane and Albert Pahlsson Foundation
- Tianjin Major Scientific and Technological Project Grant [09ZCZDSF04500]
- Early Stage Training network CaVNet
- European Union
- Juvenile Diabetes Research Foundation
- Swedish Strategic Research area in diabetes EXODIAB
- Knut and Alice Wallenberg foundation
- Novo Nordisk Fonden [NNF13OC0005771] Funding Source: researchfish
Inappropriate surface expression of voltage-gated Ca2+ channels (Ca-V) in pancreatic beta-cells may contribute to the development of type 2 diabetes. First, failure to increase intracellular Ca2+ concentrations at the sites of exocytosis impedes insulin release. Furthermore, excessive Ca2+ influx may trigger cytotoxic effects. The regulation of surface expression of Ca-V channels in the pancreatic beta-cells remains unknown. Here, we used real-time 3D confocal and TIRFM imaging, immunocytochemistry, cellular fractionation, immunoprecipitation and electrophysiology to study trafficking of L-type Ca(V)1.2 channels upon beta-cell stimulation. We found decreased surface expression of Ca(V)1.2 and a corresponding reduction in L-type whole-cell Ca2+ currents in insulin-secreting INS-1 832/13 cells upon protracted (15-30 min) stimulation. This internalization occurs by clathrin-dependent endocytosis and could be prevented by microtubule or dynamin inhibitors. eIF3e (Eukaryotic translation initiation factor 3 subunit E) is part of the protein translation initiation complex, but its effect on translation are modest and effects in ion channel trafficking have been suggested. The factor interacted with Ca(V)1.2 and regulated Ca(V)1.2 traffic bidirectionally. eIF3e silencing impaired Ca(V)1.2 internalization, which resulted in an increased intracellular Ca2+ load upon stimulation. These findings provide a mechanism for regulation of L-type Ca-V channel surface expression with consequences for beta-cell calcium homeostasis, which will affect pancreatic beta-cell function and insulin production.
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