期刊
PLOS ONE
卷 8, 期 2, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0056554
关键词
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资金
- National Institutes of Health (NIH) [P20RR021949, 5k25hl088262-04, 5R01 HL085847]
- National Science Foundation (NSF) (MRI through SC GEAR program) [CBET-0923311]
- Guangdong Provincial Department of Science and Technology, China [2011B050400011]
- State Key Laboratory of Precision Measuring Technology and Instruments (Tianjin University)
- Siebel Institute Postdoctoral Fellowship [41523-31595-44-OYZHMA-IQKEH]
- National Science Foundation (NSF) (SC EPSCoR RII through SC GEAR program) [EPS-0903795]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [923311] Funding Source: National Science Foundation
Understanding how stem cells interact with cardiomyocytes is crucial for cell-based therapies to restore the cardiomyocyte loss that occurs during myocardial infarction and other cardiac diseases. It has been thought that functional myocardial repair and regeneration could be regulated by stem cell-cardiomyocyte contact. However, because various contact modes (junction formation, cell fusion, partial cell fusion, and tunneling nanotube formation) occur randomly in a conventional coculture system, the particular regulation corresponding to a specific contact mode could not be analyzed. In this study, we used laser-patterned biochips to define cell-cell contact modes for systematic study of contact-mediated cellular interactions at the single-cell level. The results showed that the biochip design allows defined stem cell-cardiomyocyte contact-mode formation, which can be used to determine specific cellular interactions, including electrical coupling, mechanical coupling, and mitochondria transfer. The biochips will help us gain knowledge of contact-mediated interactions between stem cells and cardiomyocytes, which are fundamental for formulating a strategy to achieve stem cell-based cardiac tissue regeneration.
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