期刊
PLOS ONE
卷 8, 期 2, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0056045
关键词
-
资金
- INSERM (Institut national de la sante et de la recherche medicale)
- University of Angers
- La ligue contre le Cancer (Equipe labellisee)
IL-34 is a recently identified cytokine that signals via the M-CSF receptor and promotes monocyte survival. Depending on the environment, monocytes can differentiate into macrophages (M phi) or dendritic cells (DC). A wide spectrum of M phi and DC subsets, with distinct phenotypes and functions, has been described. To date, the phenotype of monocytes exposed to IL-34 remains unexplored. We report here that IL-34 induces the differentiation of monocytes into CD14(high) CD163(high) CD1a(-) M phi (IL-34-M phi). Upon LPS stimulation, IL-34-M phi exhibit an IL-10(high) IL-12(low) M2 profile and express low levels of the costimulatory molecules CD80 and CD86. IL-34-M phi exhibit poor T cell costimulatory properties, and have potent immunosuppressive properties (decrease of TCR-stimulated T cell proliferation). For all the parameters analyzed, IL-34-M phi are phenotypically and functionally similar to M-CSF-M phi. IL-34 appears as efficient as M-CSF in inducing the generation of immunosuppressive M phi. Moreover, the generation of IL-34-M phi is mediated through the M-CSF receptor, is independent of endogenous M-CSF consumption and is potentiated by IL-6. In an attempt to identify strategies to prevent a deleterious M2 cell accumulation in some pathological situations, we observed that IFN gamma and GM-CSF prevent the generation of immunosuppressive M phi induced by IL-34. IFN gamma also switches established IL-34-M phi into immunostimulatory M phi. In conclusion, we demonstrate that IL-34 drives the differentiation of monocytes into immunosuppressive M2, in a manner similar to M-CSF, and that IFN gamma and GM-CSF prevent this effect.
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