期刊
PLOS ONE
卷 8, 期 1, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0053543
关键词
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资金
- FEDER through COMPETE (Programa Operacional Factores de Competitividade)
- FCT project [PTDC/AGR-CFL/112996/2009]
- Human Potential Operational Programme (National Strategic Reference Framework)
- European Social Fund (EU)
- FCT [SFRH/BPD/41700/2007]
- Marie Curie Action of the European Union (FP7-PEOPLE-IEF)
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/41700/2007, PTDC/AGR-CFL/112996/2009] Funding Source: FCT
Trees necessarily experience changes in temperature, requiring efficient short-term strategies that become crucial in environmental change adaptability. DNA methylation and histone posttranslational modifications have been shown to play a key role in both epigenetic control and plant functional status under stress by controlling the functional state of chromatin and gene expression. Cork oak (Quercus suber L.) is a key stone of the Mediterranean region, growing at temperatures of 45 degrees C. This species was subjected to a cumulative temperature increase from 25 degrees C to 55 degrees C under laboratory conditions in order to test the hypothesis that epigenetic code is related to heat stress tolerance. Electrolyte leakage increased after 35 degrees C, but all plants survived to 55 degrees C. DNA methylation and acetylated histone H3 (AcH3) levels were monitored by HPCE (high performance capillary electrophoresis), MS-RAPD (methylation-sensitive random-amplified polymorphic DNA) and Protein Gel Blot analysis and the spatial distribution of the modifications was assessed using a confocal microscope. DNA methylation analysed by HPCE revealed an increase at 55 degrees C, while MS-RAPD results pointed to dynamic methylation-demethylation patterns over stress. Protein Gel Blot showed the abundance index of AcH3 decreasing from 25 degrees C to 45 degrees C. The immunohistochemical detection of 5-mC (5-methyl-2'-deoxycytidine) and AcH3 came upon the previous results. These results indicate that epigenetic mechanisms such as DNA methylation and histone H3 acetylation have opposite and particular dynamics that can be crucial for the stepwise establishment of this species into such high stress (55 degrees C), allowing its acclimation and survival. This is the first report that assesses epigenetic regulation in order to investigate heat tolerance in forest trees.
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