期刊
PLOS ONE
卷 8, 期 3, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0058679
关键词
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资金
- National Institutes of Health [GM66181]
- Canadian Institutes of Health Research [MOP 38028, MOP 79428]
- Canada Research Chair
- Fonds de Recherche du Quebec - Sante
- Natural Sciences and Engineering Council
- Ontario Students Opportunity Trust Fund
Changes in the expression of gamma-aminobutyric acid type A (GABA(A)) receptors can either drive or mediate homeostatic alterations in neuronal excitability. A homeostatic relationship between alpha 5 subunit-containing GABA(A) (alpha 5GABA(A)) receptors that generate a tonic inhibitory conductance, and HCN channels that generate a hyperpolarization-activated cation current (I-h) was recently described for cortical neurons, where a reduction in I-h was accompanied by a reciprocal increase in the expression of alpha 5GABA(A) receptors resulting in the preservation of dendritosomatic synaptic function. Here, we report that in mice that lack the alpha 5 subunit gene (Gabra5-/-), cultured embryonic hippocampal pyramidal neurons and ex vivo CA1 hippocampal neurons unexpectedly exhibited a decrease in I-h current density (by 40% and 28%, respectively), compared with neurons from wild-type (WT) mice. The resting membrane potential and membrane hyperpolarization induced by blockade of I-h with ZD-7288 were similar in cultured WT and Gabra5-/- neurons. In contrast, membrane hyperpolarization measured after a train of action potentials was lower in Gabra5-/- neurons than in WT neurons. Also, membrane impedance measured in response to low frequency stimulation was greater in cultured Gabra5-/- neurons. Finally, the expression of HCN1 protein that generates I-h was reduced by 41% in the hippocampus of Gabra5-/- mice. These data indicate that loss of a tonic GABAergic inhibitory conductance was followed by a compensatory reduction in I-h. The results further suggest that the maintenance of resting membrane potential is preferentially maintained in mature and immature hippocampal neurons through the homeostatic co-regulation of structurally and biophysically distinct cation and anion channels.
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