相关参考文献
注意：仅列出部分参考文献，下载原文获取全部文献信息。Isolation of Metarhizium anisopliae carboxypeptidase A with native disulfide bonds from the cytosol of Escherichia coli BL21(DE3)
Brian P. Austin et al.
PROTEIN EXPRESSION AND PURIFICATION (2012)
Expression of proteins in Escherichia coli as fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline
Stephen N. Hewitt et al.
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS (2011)
Double mutant MBP refolds at same rate in free solution as inside the GroEL/GroES chaperonin chamber when aggregation in free solution is prevented
Navneet K. Tyagi et al.
FEBS LETTERS (2011)
A systematic survey of in vivo obligate chaperonin-dependent substrates
Kei Fujiwara et al.
EMBO JOURNAL (2010)
Overproduction, purification and structure determination of human dual-specificity phosphatase 14
George T. Lountos et al.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY (2009)
Folding trajectories of human dihydrofolate reductase inside the GroEL-GroES chaperonin cavity and free in solution
Reto Horst et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2007)
Mutations that alter the equilibrium between open and closed conformations of Escherichia coli maltose-binding protein impede its ability to enhance the solubility of passenger proteins
Sreedevi Nallamsetty et al.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2007)
Over-expression and refolding of MAP kinase phosphatase 3
John K. Mark et al.
PROTEIN EXPRESSION AND PURIFICATION (2007)
N-terminal domains of native multidomain proteins have the potential to assist de novo folding of their downstream domains in vivo by acting as solubility enhancers
Chul Woo Kim et al.
PROTEIN SCIENCE (2007)
Overproduction, purification, and biochemical characterization of the dual specificity H1 protein phosphatase encoded by variola major virus
Joseph E. Tropea et al.
PROTEIN EXPRESSION AND PURIFICATION (2006)
Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection
Tomoya Baba et al.
MOLECULAR SYSTEMS BIOLOGY (2006)
Gateway vectors for the production of combinatorially-tagged His6-MBP fusion proteins in the cytoplasm and periplasm of Escherichia Coli
S Nallamsetty et al.
PROTEIN SCIENCE (2005)
Large-scale protein expression for proteome research
U Korf et al.
PROTEOMICS (2005)
Escherichia coli fusion carrier proteins act as solubilizing agents for recombinant uncoupling protein 1 through interactions with GroEL
P Douette et al.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2005)
A universal strategy for stable intracellular antibodies
S Shaki-Loewenstein et al.
JOURNAL OF IMMUNOLOGICAL METHODS (2005)
Proteome-wide analysis of chaperonin-dependent protein folding in Escherichia coli
MJ Kerner et al.
CELL (2005)
Making the most of affinity tags
DS Waugh
TRENDS IN BIOTECHNOLOGY (2005)
Heterologous gene expression using self-assembled supra-molecules with high affinity for HSP70 chaperone
JY Ahn et al.
NUCLEIC ACIDS RESEARCH (2005)
Protein aggregation during overexpression limited by peptide extensions with large net negative charge
YB Zhang et al.
PROTEIN EXPRESSION AND PURIFICATION (2004)
Efficient site-specific processing of fusion proteins by tobacco vein mottling virus protease in vivo and in vitro
S Nallamsetty et al.
PROTEIN EXPRESSION AND PURIFICATION (2004)
Insights into the conformational equilibria of maltose-binding protein by analysis of high affinity mutants
PG Telmer et al.
JOURNAL OF BIOLOGICAL CHEMISTRY (2003)
Maltodextrin-binding proteins from diverse bacteria and archaea are potent solubility enhancers
JD Fox et al.
FEBS LETTERS (2003)
High-throughput screening of soluble recombinant proteins
YP Shih et al.
PROTEIN SCIENCE (2002)
Rapid screening for improved solubility of small human proteins produced as fusion proteins in Escherichia coli
M Hammarström et al.
PROTEIN SCIENCE (2002)
Directed evolution of substrate-optimized GroEL/S chaperonins
JD Wang et al.
CELL (2002)
The P1′ specificity of tobacco etch virus protease
RB Kapust et al.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2002)
Proteome-scale purification of human proteins from bacteria
P Braun et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2002)
Tobacco etch virus protease:: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency
RB Kapust et al.
PROTEIN ENGINEERING (2001)
GroEL/GroES-mediated folding of a protein too large to be encapsulated
TK Chaudhuri et al.
CELL (2001)
Formation of soluble inclusion bodies by HPV E6 oncoprotein fused to maltose-binding protein
Y Nominé et al.
PROTEIN EXPRESSION AND PURIFICATION (2001)
Escherichia coli maltose-binding protein as a molecular chaperone for recombinant intracellular cytoplasmic single-chain antibodies
H Bach et al.
JOURNAL OF MOLECULAR BIOLOGY (2001)
A strategy for optimizing the monodispersity of fusion proteins:: application to purification of recombinant HPV E6 oncoprotein
Y Nominé et al.
PROTEIN ENGINEERING (2001)
Single amino acid substitutions on the surface of Escherichia coli maltose-binding protein can have a profound impact on the solubility of fusion proteins
JD Fox et al.
PROTEIN SCIENCE (2001)
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
KA Datsenko et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2000)
分享论文
