期刊
PLOS ONE
卷 7, 期 12, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0051314
关键词
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资金
- National Science Foundation (NSF)
- Natural Sciences and Engineering Research Council (NSERC)
- Canada Research Chair in Bioanalytical Chemistry
- European Molecular Biology Organization
- National Institutes of Health [GM77856, GM084716]
- National Cancer Institute (NCI) Physical Sciences Oncology program [U54CA143836]
- Department of Energy (DOE) Office of Basic Energy Sciences (FWP SISGRKN)
- Office of Science, of the U.S. Department of Energy [DE-AC02-05CH11231]
- National Centre of Competence in Research (NCCR) Neural Plasticity and Repair
- Holcim Foundation
- National Competence Center in Biomedical Imaging (NCCBI)
Recent advances in fluorescence microscopy have extended the spatial resolution to the nanometer scale. Here, we report an engineered photoconvertible fluorescent protein (pcFP) variant, designated as mMaple, that is suited for use in multiple conventional and super-resolution imaging modalities, specifically, widefield and confocal microscopy, structured illumination microscopy (SIM), and single-molecule localization microscopy. We demonstrate the versatility of mMaple by obtaining super-resolution images of protein organization in Escherichia coli and conventional fluorescence images of mammalian cells. Beneficial features of mMaple include high photostability of the green state when expressed in mammalian cells and high steady state intracellular protein concentration of functional protein when expressed in E. coli. mMaple thus enables both fast live-cell ensemble imaging and high precision single molecule localization for a single pcFP-containing construct.
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