期刊
PLOS ONE
卷 7, 期 9, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0043045
关键词
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资金
- Israel Science Foundation (ISF) [204-10, 1331/11]
- United States-Israel Agricultural Research and Development Fund (BARD) Research grant [IS-4229-09]
- Eshkol Foundation
- Israeli Ministry of Science
- Edmond J. Safra Bioinformatics program at Tel-Aviv University
A ring of 8-15 identical c-subunits is essential for ion-translocation by the rotary electromotor of the ubiquitous FOF1-ATPase. Here we present the crystal structure at 3.4 angstrom resolution of the c-ring from chloroplasts of a higher plant (Pisum sativum), determined using a native preparation. The crystal structure was found to resemble that of an (ancestral) cyanobacterium. Using elastic network modeling to investigate the ring's eigen-modes, we found five dominant modes of motion that fell into three classes. They revealed the following deformations of the ring: (I) ellipsoidal, (II) opposite twisting of the luminal circular surface of the ring against the stromal surface, and (III) kinking of the hairpin-shaped monomers in the middle, resulting in bending/stretching of the ring. Extension of the elastic network analysis to rings of different cn-symmetry revealed the same classes of dominant modes as in P. sativum (c(14)). We suggest the following functional roles for these classes: The first and third classes of modes affect the interaction of the c-ring with its counterparts in F-O, namely subunits a and bb'. These modes are likely to be involved in ion-translocation and torque generation. The second class of deformation, along with deformations of subunits gamma and epsilon might serve to elastically buffer the torque transmission between F-O and F-1.
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