期刊
PLOS ONE
卷 7, 期 4, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0034983
关键词
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资金
- Fundacao para a Ciencia e a Tecnologia (FCT) [SFRH/BD/36378/2007, SFRH/BD/16954/2004, SFRH/BPD/64095/2009, PTDC/BIA-MIC/100370/2008]
- European Science Foundation (ESF) (III Quadro Comunitario de Apoio)
- Programa Operacional Factores de Competitividade (COMPETE) in it's FEDER (Fundo Europeu de Desenvolvimento Regional) component
- Fundação para a Ciência e a Tecnologia [SFRH/BD/16954/2004, SFRH/BPD/64095/2009, SFRH/BD/36378/2007, PTDC/BIA-MIC/100370/2008] Funding Source: FCT
Cyanobacteria are a group of photosynthetic prokaryotes that have a diverse morphology, minimal nutritional requirements and metabolic plasticity that has made them attractive organisms to use in biotechnological applications. The use of these organisms as cell factories requires the knowledge of their physiology and metabolism at a systems level. For the quantification of gene transcripts real-time quantitative polymerase chain reaction (RT-qPCR) is the standard technique. However, to obtain reliable RT-qPCR results the use and validation of reference genes is mandatory. Towards this goal we have selected and analyzed twelve candidate reference genes from three morphologically distinct cyanobacteria grown under routinely used laboratory conditions. The six genes exhibiting less variation in each organism were evaluated in terms of their expression stability using geNorm, NormFinder and BestKeeper. In addition, the minimum number of reference genes required for normalization was determined. Based on the three algorithms, we provide a list of genes for cyanobacterial RT-qPCR data normalization. To our knowledge, this is the first work on the validation of reference genes for cyanobacteria constituting a valuable starting point for future works.
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