期刊
PLOS ONE
卷 7, 期 3, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0033163
关键词
-
资金
- Canadian Institutes for Health Research (CIHR) [MOP-26 84205]
- Heart and Stroke Foundation of British Columbia and Yukon (HSFBCY) [20R20069]
- Natural Sciences and Engineering Research Council of Canada
- CIHR
- Canadian Lung Association
- Integrated and Mentored Pulmonary and Cardiovascular Training (IMPACT)
- Michael Smith Foundation for Health Research (MSFHR)
Objective: Granzyme B (GrB) is a pro-apoptotic serine protease that contributes to immune-mediated target cell apoptosis. However, during inflammation, GrB accumulates in the extracellular space, retains its activity, and is capable of cleaving extracellular matrix (ECM) proteins. Recent studies have implicated a pathogenic extracellular role for GrB in cardiovascular disease, yet the pathophysiological consequences of extracellular GrB activity remain largely unknown. The objective of this study was to identify proteoglycan (PG) substrates of GrB and examine the ability of GrB to release PG-sequestered TGF-beta 1 into the extracellular milieu. Methods/Results: Three extracellular GrB PG substrates were identified; decorin, biglycan and betaglycan. As all of these PGs sequester active TGF-beta 1, cytokine release assays were conducted to establish if GrB-mediated PG cleavage induced TGF-beta 1 release. Our data confirmed that GrB liberated TGF-beta 1 from all three substrates as well as from endogenous ECM and this process was inhibited by the GrB inhibitor 3,4-dichloroisocoumarin. The released TGF-beta 1 retained its activity as indicated by the induction of SMAD-3 phosphorylation in human coronary artery smooth muscle cells. Conclusion: In addition to contributing to ECM degradation and the loss of tissue structural integrity in vivo, increased extracellular GrB activity is also capable of inducing the release of active TGF-beta 1 from PGs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据