期刊
PLOS ONE
卷 7, 期 1, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0029597
关键词
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资金
- European Commission [LSHG-CT-2006-018739]
- ESTOOLS [222943]
- Neurostemcell [LSHB-CT-2007-037766]
- UK Medical Research Council
- German Research Foundation (DFG) [SFB-TR3]
- German Federal Ministry for Education and Research (BMBF) [01GNO813]
- Stem Cell Network North Rhine Westphalia [L-072.0055]
- Hertie Foundation
- Swedish Research Council
- Swedish Hjarnfonden
- EMBO
- MRC [G0800784, G1001028] Funding Source: UKRI
- Medical Research Council [G0800784, MC_PC_12009, G1100526, G1001028, G0800784B] Funding Source: researchfish
- The Brain Tumour Charity [8/105] Funding Source: researchfish
Human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) provide new prospects for studying human neurodevelopment and modeling neurological disease. In particular, iPSC-derived neural cells permit a direct comparison of disease-relevant molecular pathways in neurons and glia derived from patients and healthy individuals. A prerequisite for such comparative studies are robust protocols that efficiently yield standardized populations of neural cell types. Here we show that long-term self-renewing neuroepithelial-like stem cells (lt-NES cells) derived from 3 hESC and 6 iPSC lines in two independent laboratories exhibit consistent characteristics including i) continuous expandability in the presence of FGF2 and EGF; ii) stable neuronal and glial differentiation competence; iii) characteristic transcription factor profile; iv) hindbrain specification amenable to regional patterning; v) capacity to generate functionally mature human neurons. We further show that lt-NES cells are developmentally distinct from fetal tissue-derived radial glia-like stem cells. We propose that lt-NES cells provide an interesting tool for studying human neurodevelopment and may serve as a standard system to facilitate comparative analyses of hESC and hiPSC-derived neural cells from control and diseased genetic backgrounds.
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