期刊
PLOS ONE
卷 6, 期 12, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0029160
关键词
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资金
- Korea Research Foundation
- Korean Government (MOEHRD) [KRF-2008-314-E00214]
- National Research Foundation of Korea through the Oromaxillofacial Dysfunction Research Center for the Elderly at Seoul National University at Korea [2011-0028234]
Transforming growth factor-beta 1 (TGF-beta 1) signaling plays a key role in vertebrate development, homeostasis, and disease. Nuclear factor I-C (NFI-C) has been implicated in TGF-beta 1 signaling, extracellular matrix gene transcription, and tooth root development. However, the functional relationship between NFI-C and TGF-beta 1 signaling remains uncharacterized. The purpose of this study was to identify the molecular interactions between NFI-C and TGF-beta 1 signaling in mouse odontoblasts. Real-time polymerase chain reaction and western analysis demonstrated that NFI-C expression levels were inversely proportional to levels of TGF-beta 1 signaling molecules during in vitro odontoblast differentiation. Western blot and immunofluorescence results showed that NFI-C was significantly degraded after TGF-beta 1 addition in odontoblasts, and the formation of the Smad3 complex was essential for NFI-C degradation. Additionally, ubiquitination assay results showed that Smurf1 and Smurf2 induced NFI-C degradation and polyubiquitination in a TGF-beta 1-dependent manner. Both kinase and in vitro binding assays revealed that the interaction between NFI-C and Smurf1/Smurf2 requires the activation of the mitogen-activated protein kinase pathway by TGF-beta 1. Moreover, degradation of NFI-C induced by TGF-beta 1 occurred generally in cell types other than odontoblasts in normal human breast epithelial cells. In contrast, NFI-C induced dephosphorylation of p-Smad2/3. These results show that crosstalk between NFI-C and TGF-beta 1 signaling regulates cell differentiation and homeostatic processes in odontoblasts, which might constitute a common cellular mechanism.
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