期刊
PLOS ONE
卷 6, 期 10, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0025421
关键词
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资金
- Consiglio Nazionale delle Ricerche, Rome, Italy
- Departamento de Investigaciones Cientificas y Tecnologicas (DICYT) at Universidad de Santiago de Chile
- International Foundation for Science [F/3115-1, C/2807-2]
- FONDECYT [N 1090723]
The antifungal effect of three furyl compounds closely related to resveratrol, (E)-3,4,5-trimethoxy-beta-(2-furyl)-styrene (1), (E)-4-methoxy-beta-(2-furyl)-styrene (2) and (E)-3,5-dimethoxy-beta-(2-furyl)-styrene (3) against Botrytis cinerea was analyzed. The inhibitory effect, at 100 mu g ml(-1) 21 of compounds 1, 2, 3 and resveratrol on conidia germination, was determined to be about 70%, while at the same concentration pterostilbene (a dimethoxyl derivative of resveratrol) produced complete inhibition. The title compounds were more fungitoxic towards in vitro mycelial growth than resveratrol and pterostilbene. Compound 3 was the most active and a potential explanation of this feature is given using density functional theory (DFT) calculations on the demethoxylation/demethylation process. Compound 3 was further evaluated for its effects on laccase production, oxygen consumption and membrane integrity of B. cinerea. An increase of the laccase activity was observed in the presence of compound 3 and, using Sytox Green nucleic acid stain, it was demonstrated that this compound altered B. cinerea membrane. Finally, compound 3 partially affected conidia respiration.
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