期刊
PLOS ONE
卷 6, 期 10, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0026780
关键词
-
资金
- University of Oslo
- Halvor Hoies Fond
- Norwegian Cancer Society
- Forskerlinjen fellowship
Background: Natural killer (NK) cells have both cytolytic and immunoregulatory functions. We recently described that these cells release the inflammatory cytokines IL-17 and IFN-gamma. However, the precise identity of the NK cell subset(s) that secrete these cytokines is not known. Methodology/Principal Findings: To isolate the cells secreting IL-17 and IFN-gamma, we took advantage of the findings that Th17/Th1 cells express chemokine receptors. Therefore, CD56(+) NK cells were stained with antibodies against various chemokine receptors and intracellularly with antibodies toward IL-17 and IFN-gamma. Consequently, we identified previously unrecognized subset of NK cells generated from normal human peripheral blood after activation with IL-2 but not PMA plus ionomycin. The cells are characterized by the expression of CD56(+) and CCR4(+), produce IL-17 and IFN-gamma and are consequently named NK17/NK1 cells. They also express CD161, NKp30, NKp44, NKp46, NKG2D, CD158, CCL22, IL-2R beta and the common gamma chain but not CD127 or IL-23R. Further, they possess T-bet and ROR gamma t transcription factors. Antibodies to IL-1 beta, IL-6, IL-21, or TGF-beta 1 do not inhibit IL-2-induced generation of NK17/NK1 cells, suggesting that IL-2 has the capacity to polarize these cells. Notably, NK17/NK1 cells are abundant in the cerebrospinal fluid (CSF) of patients with multiple sclerosis (MS) without activation, and are generated from the peripheral blood of these patients after activation with IL-2. Conclusions/Significance: NK17/NK1 cells identified here have not been previously described in healthy or MS patients.
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