期刊
PLOS ONE
卷 5, 期 2, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0009037
关键词
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资金
- Sigrid Juselius Foundation
- National Graduate School in Informational and Structural Biology
- Frontier Funding of VTT-Technical Research Centre of Finland,
- Helsinki University Central Hospital [TYH7111]
Background: Allergen-mediated cross-linking of IgE antibodies bound to the Fc epsilon RI receptors on the mast cell surface is the key feature of the type I allergy. If an allergen is a homodimer, its allergenicity is enhanced because it would only need one type of antibody, instead of two, for cross-linking. Methodology/Principal Findings: An analysis of 55 crystal structures of allergens showed that 80% of them exist in symmetric dimers or oligomers in crystals. The majority are transient dimers that are formed at high protein concentrations that are reached in cells by colocalization. Native mass spectrometric analysis showed that native allergens do indeed form transient dimers in solution, while hypoallergenic variants of them exist almost solely in the monomeric form. We created a monomeric Bos d 5 allergen and show that it has a reduced capability to induce histamine release. Conclusions/Significance: The results suggest that dimerization would be a very common and essential feature for allergens. Thus, the preparation of purely monomeric variants of allergens could open up novel possibilities for specific immunotherapy.
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