相关参考文献
注意:仅列出部分参考文献,下载原文获取全部文献信息。Sequence-independent construction of ordered combinatorial libraries with predefined crossover points
Laetitia Jezequel et al.
BIOTECHNIQUES (2008)
A high-throughput and single-tube recombination of crude PCR products using a DNA polymerase inhibitor and type IIS restriction enzyme
Ippei Kotera et al.
JOURNAL OF BIOTECHNOLOGY (2008)
A One Pot, One Step, Precision Cloning Method with High Throughput Capability
Carola Engler et al.
PLOS ONE (2008)
Developments in directed evolution for improving enzyme functions
S. Sen et al.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2007)
Biocatalytic conversion of avermectin to 4-oxo-avermectin:: Improvement of cytochrome P450 monooxygenase specificity by directed evolution
Axel Trefzer et al.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2007)
Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC
Mamie Z. Li et al.
NATURE METHODS (2007)
Enzymatic ligation assisted by nucleases: simultaneous ligation and digestion promote the ordered assembly of DNA
Gregory J. Cost
NATURE PROTOCOLS (2007)
Mutant library construction in directed molecular evolution
Tian-Wen Wang et al.
MOLECULAR BIOTECHNOLOGY (2006)
A general one-step method for the cloning of PCR products
A Bolchi et al.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY (2005)
Multivalent avimer proteins evolved by exon shuffling of a family of human receptor domains
J Silverman et al.
NATURE BIOTECHNOLOGY (2005)
Multiplex-PCR-based recombination as a novel high-fidelity method for directed evolution
T Eggert et al.
CHEMBIOCHEM (2005)
Novel methods for directed evolution of enzymes: quality, not quantity
S Lutz et al.
CURRENT OPINION IN BIOTECHNOLOGY (2004)
In planta engineering of viral RNA replicons:: Efficient assembly by recombination of DNA modules delivered by Agrobacterium
S Marillonnet et al.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2004)
Maize (Zea mays)-derived bovine trypsin:: characterization of the first large-scale, commercial protein product from transgenic plants
SL Woodard et al.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY (2003)
General method for sequence-independent site-directed chimeragenesis
K Hiraga et al.
JOURNAL OF MOLECULAR BIOLOGY (2003)
Synthetic shuffling expands functional protein diversity by allowing amino acids to recombine independently
JE Ness et al.
NATURE BIOTECHNOLOGY (2002)
Growth factor engineering by degenerate homoduplex gene family recombination
WM Coco et al.
NATURE BIOTECHNOLOGY (2002)
A novel, high performance enzyme for starch liquefaction -: Discovery and optimization of a low pH, thermostable α-amylase
TH Richardson et al.
JOURNAL OF BIOLOGICAL CHEMISTRY (2002)
Libraries of hybrid proteins from distantly related sequences
V Sieber et al.
NATURE BIOTECHNOLOGY (2001)
DNA shuffling method for generating highly recombined genes and evolved enzymes
WM Coco et al.
NATURE BIOTECHNOLOGY (2001)