期刊
PLOS ONE
卷 3, 期 8, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0002966
关键词
-
资金
- Juvenile Diabetes Research Foundation [postdoctoral fellow]
- National Institute for Health Research (NIHR)
- Cambridge Biomedical Research Centre
- Wellcome Trust [079895]
- British Heart Foundation [FS/05/061/19501]
- Scientifical and Technical Research Council of Turkey [TUBITAK-SBAG3334]
- International Centre for Genetic Engineering and Biotechnology [ICGEB-CRP/TUR04-01]
Lymphoblastoid cell lines (LCL) are being actively and extensively used to examine the expression of specific genes and genome-wide expression profiles, including allele specific expression assays. However, it has recently been shown that approximately 10% of human genes exhibit random patterns of monoallelic expression within single clones of LCLs. Consequently allelic imbalance studies could be significantly compromised if bulk populations of donor cells are clonal, or near clonal. Here, using X chromosome inactivation as a readout, we confirm and quantify widespread near monoclonality in two independent sets of cell lines. Consequently, we recommend where possible the use of bulk, non cell line, ex vivo cells for allele specific expression assays.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据