期刊
PLATELETS
卷 21, 期 3, 页码 211-220出版社
INFORMA HEALTHCARE
DOI: 10.3109/09537100903528269
关键词
Curcumin; Syk; PLC gamma 2; GPVI; platelet activation
资金
- National Institutes of Health [HL81322, HL80444, HL60683]
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL081322, R01HL060683, R01HL080444] Funding Source: NIH RePORTER
Turmeric (Curcuma longa), a herbal remedy and culinary spice, has been used in traditional Indian culture for millennia. An active ingredient found in turmeric is curcumin (diferuloylmethane). In the current study, we investigated the antiplatelet properties of this naturally occurring compound. Curcumin inhibited human platelet aggregation and dense granule secretion induced by GPVI agonist convulxin in a concentration-dependent manner. At 50 mu M, it effectively inhibited the maximal extent of aggregation and dense granule secretion to as much as 75%. It also dramatically inhibited the activation-dependent tyrosine phosphorylation of Y753 and Y759 on PLC gamma 2, but did not affect the phosphorylation of Y145 residue on the cytosolic adaptor protein SLP-76. Interestingly, curcumin had no significant effect on the phosphorylation of Y525/Y526 present on the activation loop of Syk (spleen tyrosine kinase), but had a significant inhibitory effect on in vitro Syk kinase activity. Moreover, the inhibitory action of curcumin is not due to an inhibition of thromboxane generation because all our studies were performed using aspirin-treated platelets. We conclude that curcumin inhibits platelet activation induced by GPVI agonists through interfering with the kinase activity of Syk and the subsequent activation of PLC gamma 2.
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