期刊
PLASTIC AND RECONSTRUCTIVE SURGERY
卷 129, 期 1, 页码 46E-54E出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/PRS.0b013e3182362010
关键词
-
类别
资金
- Fond National Suisse [310030_120571]
- Heart and Stroke Foundation Ontario [NA7086]
- Canadian Institutes of Health Research [210820]
- Swiss National Science Foundation (SNF) [310030_120571] Funding Source: Swiss National Science Foundation (SNF)
Background: Platelet preparations for tissue healing are usually preactivated before application to deliver concentrated growth factors. In this study, the authors investigated the differences between nonactivated and thrombin-activated platelets in wound healing. Methods: The healing effects (i.e., wound closure, myofibroblast formation, and angiogenesis) of nonactivated and thrombin-activated platelets were compared in experimental wounds in diabetic (db/db) animals. In vitro, fibroblast phenotype and function were tested in response to platelets and activated platelets. No treatment served as a negative control. Results: Wounds treated with platelets reached 90 percent closure after 15 days, faster than activated platelets (26 days), and with higher levels of myofibroblasts and angiogenesis. In vitro, platelets enhanced cell migration and induced twofold higher myofibroblast differentiation and contraction compared with activated platelets. Conclusions: Platelets stimulate wound healing more efficiently compared with activated platelets by enhancing fibroblast differentiation and contractile function. Similar levels of growth factors may induce different biological effects when delivered on demand rather than in an initial bolus. (Plast. Reconstr. Surg. 129: 46e, 2012.)
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据