期刊
PLASMONICS
卷 8, 期 2, 页码 803-810出版社
SPRINGER
DOI: 10.1007/s11468-012-9476-8
关键词
UO22+; DNAzyme; Nanogold; Rhodamine 6G; Surface-enhanced resonance Raman scattering
资金
- National Natural Science Foundation of China [21267004]
- Research Funds of Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry
- Research Funds of Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology
In pH5.5 2-(N-morpholino)-ethanosulfonic acid buffer solution containing 0.25 M NaCl at 80 degrees C, the single-stranded substrate DNA hybridizes with the enzyme DNA to form double-stranded DNA (dsDNA). The substrate chain of dsDNA could be cracked catalytically by UO22+ to produce a short single-stranded DNA (ssDNA) that adsorbed on the nanogold (NG) surface to form a stable nanogold-ssDNA conjugate and then further combine with rhodamine 6G (RhG) to form a NG-ssDNA-RhG conjugate that can be monitored by the surface-enhanced resonance Raman scattering (SERRS) spectral technique at 1,360 cm(-1). Under the selected conditions, the increased SERRS intensity Delta I-1360 was linear to UO22+ concentration in the range of 5-125 nmol/L, with a detection limit of 1.6 nmol/L. Using a 0.5-mu mol/L Hg2+ as enhancer, a 2.5-100-nmol/L UO22+ can be determined.
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