期刊
PLASMONICS
卷 7, 期 2, 页码 185-190出版社
SPRINGER
DOI: 10.1007/s11468-011-9292-6
关键词
UO22+; Catalytic aptamer cracking; Nanogold catalysis; Resonance scattering spectral assay
资金
- National Natural Science Foundation of China [21075023, 20865002, 20965002]
- Natural Science Foundation of Guangxi [0991021z]
- Research Funds of Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education
In pH 5.5 2-(N-morpholino)-ethanosulfonic acid buffer solution containing 0.0125 M NaCl at 80 A degrees C, the single-stranded substrate DNA hybrid with enzyme DNA to form double-stranded DNA (dDNA). The substrate chain of dDNA could be cracked catalytically by UO (2) (2+) to produce a short single-stranded DNA (ssDNA) that adsorbed on the nanogold (NG) surface to form stable NGssDNA conjugate, and the unadsorbed NG take place aggregation to produce the NG aggregations in blue color. Both NG and NGssDNA exhibited strong catalytic activity on the gold particle reaction between HAuCl4 and ascorbic acid that can be monitored by resonance scattering (RS) spectral technique at 620 nm. However, the catalytic effect of NG aggregation was very weak and it cannot be separated from the cracked reaction solution. When the UO (2) (2+) concentration increased, the ssDNA increased, the NGssDNA increased, the formed gold particles increased, and the RS intensity at 620 nm increased. The increased RS intensity Delta I (620 nm) was linear to UO (2) (2+) concentration in the range of 3.35-23.45 pM, with a regression equation of Delta I (620 nm) = 27.6C + 29.1, and detection limit of 0.1 pM. This new RS assay was applied to analysis of UO (2) (2+) in water sample with satisfactory results.
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