期刊
PLASMID
卷 75, 期 -, 页码 12-17出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.plasmid.2014.07.001
关键词
Multiprotein expression systems; Cell biology; Structural biology
资金
- Swiss National Science Foundation [31003A_146975]
- Swiss National Science Foundation (SNF) [31003A_146975] Funding Source: Swiss National Science Foundation (SNF)
Multigene delivery systems for heterologous multiprotein expression in mammalian cells are a key technology in contemporary biological research. Multiprotein expression is essential for a variety of applications, including multiparameter analysis of living cells in vitro, changing the fate of stem cells, or production of multiprotein complexes for structural biology. Depending on the application, these expression systems have to fulfill different requirements. For some applications, homogenous expression in all cells with defined stoichiometry is necessary, whereas other applications need long term expression or require that the proteins are not modified at the N- and C-terminus. Here we summarize available multiprotein expression systems and discuss their advantages and disadvantages. (C) 2014 Elsevier Inc. All rights reserved.
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