期刊
PLANTA MEDICA
卷 80, 期 17, 页码 1622-1627出版社
GEORG THIEME VERLAG KG
DOI: 10.1055/s-0034-1383049
关键词
Ziziphus jujuba; Rhamnaceae; erythropoietin; hypoxia response element; hypoxia-inducible factor
资金
- Hong Kong Research Grants Council Theme-based Research Scheme [T13-607/12R]
- GRF [662911, 663012, 662713, M-HKUST604/13]
- Hong Kong Jockey Club Charities Trust
- Foundation of The Awareness of Nature [TAON12SC01]
- ITC [GHP/059/12SZ]
- Hong Kong Chiu Chow Chamber of Commerce
The fruit of Ziziphus jujuba Mill., known as jujube or Chinese date, is commonly consumed as health supplement or herbal medicine worldwide. To study the beneficial role of jujube in enhancing hematopoietic function, we investigated its roles on the expression of erythropoietin in cultured Hep3B human hepatocellular carcinoma cells. Application of chemically standardized jujube water extract stimulated erythropoietin expression in a dose-dependent manner, with the highest response by similar to 100% of increase. A plasmid containing hypoxia response element, a critical regulator for erythropoietin transcription, was transfected into Hep3B cells. Application of jujube water extract onto the transfected cells induced the transcriptional activity of the hypoxia response element. To account for its transcriptional activation, the expression of hypoxia-inducible factor-1 was increased after treatment with jujube water extract: the increase was in both mRNA and protein levels. These results confirmed the hematopoietic function of jujube in the regulation of erythropoietin expression in liver cells.
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