期刊
PLANTA MEDICA
卷 77, 期 7, 页码 765-770出版社
GEORG THIEME VERLAG KG
DOI: 10.1055/s-0030-1250566
关键词
Trigonella foenum-graecum L.; Leguminosae; chalcone isomerase; Arabidopsis; tt5
资金
- Program for New Century Excellent Talents in University [NCE09-0423]
- Ministry of Education of the People's Republic of China
- National Natural Science Foundation of China [NSFC-31000149]
- Key Projects in the National Science & Technology Pillar Program [2006BAD08A08]
- Basic Research Program [20060544]
- Science and Technology Department of Jilin Province
- International Technology Cooperation Project, Changchun, China [06GH07]
Flavonoids belong to a group of plant natural products with variable phenolic structures and play important roles in protection against biotic and abiotic stress. Fenugreek (Trigonella foenum-graecum L.) seeds and stems contain flavonol glycosides and isoflavone derivatives. Up to now, the molecular features of fenugreek flavonoid biosynthesis have not been characterized. Here we present cloning of a cDNA encoding a chalcone isomerase (namely TFGCHI-1) from the leaves of T. foenum-graecum which convert chalcones to flavanones in vitro. Transformation of Arabidopsis loss-of-function tt5 (chi) mutant with a TFGCHI-1 cDNA complemented tt5 and produced higher levels of flavonol glycosides than wild-type Col-0.
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