4.5 Article

Cajanol Inhibits the Growth of Escherichia coli and Staphylococcus aureus by Acting on Membrane and DNA Damage

期刊

PLANTA MEDICA
卷 77, 期 2, 页码 158-163

出版社

GEORG THIEME VERLAG KG
DOI: 10.1055/s-0030-1250146

关键词

Cajanus cajan (L.) Millsp.; pigeon pea; cajanol; lecithin; phosphate groups; DNA

资金

  1. National Natural Science Foundation of China [30770231]
  2. Heilongjiang Province Science Foundation for Excellent Youths [JC200704]
  3. Agricultural Science and Technology Achievements Transformation Fund [2009GB23600514]
  4. Chinese Ministry of Education [108049, MS2010DBLY031]
  5. Innovative Program for Importation of International Advanced Agricultural Science and Technology, National Forestry Bureau [2006-4-75]
  6. Key Program for Science and Technology Development of Harbin [2009AA3BS083]
  7. Research Foundation for Science, Technology Innovation Talents of Harbin [2006RFXXS001]
  8. Fundamental Research Funds for the Central Universities [DL09EA04]
  9. National Key Technology R D Program [2006BAD18B0405]
  10. Northeast Forestry University
  11. NEFU Foundation [GRAP09]

向作者/读者索取更多资源

In the present study, the mechanism of antibacterial activity of cajanol extracted from the roots of Cajanus cajan (L.) Millsp. towards Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) was investigated. The antibacterial activity of cajanol was evaluated towards six bacterial strains (Staphylococcus epidermidis, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Proteus vulgaris, and Pseudomonas aeruginosa) by the broth microdilution method. It showed strong antibacterial activity towards all bacteria tested with minimal inhibition concentration (MIC) values ranging from 98.90 mu M to 197.8 mu M. Cajanol-induced death rates in the most sensitive strains (E. coli, 96.55% and S. aureus, 97.25%) were analyzed by flow cytometry. Furthermore, the activity of cajanol on the membranes of E. coli and S. aureus was investigated by using lecithin, phosphate groups, and fluorescence microscopy. Cajanol-induced DNA damage was observed by agarose gel electrophoresis. In summary, cajanol inhibited E. coli only by DNA damage, whereas S. aureus was inhibited by affecting both, the lecithin and phosphate groups on the cellular membrane and DNA. The present study shows that cajanol possesses antibacterial activity in vitro towards both gram-negative and gram-positive bacteria and therefore may be a promising candidate as an antibacterial agent for the therapy of microbial infections.

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