4.7 Article

β-(1,4)-Galactan remodelling in Arabidopsis cell walls affects the xyloglucan structure during elongation

期刊

PLANTA
卷 249, 期 2, 页码 351-362

出版社

SPRINGER
DOI: 10.1007/s00425-018-3008-5

关键词

beta-Galactosidases; Growth; Pectin

资金

  1. Spanish Ministerio de Economia y Competitividad (MINECO) [BFU2013-44793-P]
  2. Programa Predoctoral de Formacion de Personal Investigador grant from the Basque Government
  3. National Science Foundation (NSF) Plant Genome Program [DBI-0421683]
  4. Junta de Castilla-Leon [SA027G18]

向作者/读者索取更多资源

Main conclusionGalactan turnover occurs during cell elongation and affects the cell wall xyloglucan structure which is involved in the interaction between cellulose and xyloglucan.-(1,4)-Galactan is one of the main side chains of rhamnogalacturonan I. Although the specific function of this polymer has not been completely established, it has been related to different developmental processes. To study -(1,4)-galactan function, we have generated transgenic Arabidopsis plants overproducing chickpea I-Gal -galactosidase under the 35S CaMV promoter (35S::I-Gal) to reduce galactan side chains in muro. Likewise, an Arabidopsis double loss-of-function mutant for BGAL1 and BGAL3 Arabidopsis -galactosidases (bgal1/bgal3) has been obtained to increase galactan levels. The characterization of these plants has confirmed the role of -(1,4)-galactan in cell growth, and demonstrated that the turnover of this pectic side chain occurs during cell elongation, at least in Arabidopsis etiolated hypocotyls and floral stem internodes. The results indicate that BGAL1 and BGAL3 -galactosidases act in a coordinate way during cell elongation. In addition, this work indicates that galactan plays a role in the maintenance of the cell wall architecture during this process. Our results point to an involvement of the -(1,4)-galactan in the xyloglucan structure and the interaction between cellulose and xyloglucan.

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