期刊
PLANTA
卷 236, 期 5, 页码 1485-1498出版社
SPRINGER
DOI: 10.1007/s00425-012-1698-7
关键词
Disease resistance; Rhizoctonia solani; Magnaporthe grisea; Transcription factor; WRKY30; Oryza sativa
资金
- National Natural Science Foundation of China [30771387, 31171803]
- Commonweal Research Program of Agricultural Science of China [nyhyzx3-16]
- Research Foundation of Education Bureau of Hunan Province, China [06B027]
- Hunan Provincial Natural Science Foundation of China [10JJ2030]
WRKY transcription factors are crucial regulatory components of plant responses to pathogen infection. In the present study, we report isolation and functional characterization of the pathogen-responsive rice WRKY30 gene, whose transcripts accumulate rapidly in response to salicylic acid (SA) and jasmonic acid (JA) treatment. Overexpression of WRKY30 in rice enhanced resistance to rice sheath blight fungus Rhizoctonia solani and blast fungus Magnaporthe grisea. The enhanced resistance in the transgenic lines overexpressing WRKY30 was associated with activated expression of JA synthesis-related genes LOX, AOS2 and pathogenesis-related (PR)3 and PR10, and increased endogenous JA accumulation under the challenge of fungal pathogens. WRKY30 was nuclear-localized and had transcriptional activation ability in yeast cells, supporting that it functions as a transcription factor. Together, our findings indicate that JA plays a crucial role in the WRKY30-mediated defense responses to fungal pathogens, and that the rice WRKY30 seems promising as an important candidate gene to improve disease resistance in rice.
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