期刊
PLANTA
卷 231, 期 6, 页码 1439-1458出版社
SPRINGER
DOI: 10.1007/s00425-010-1138-5
关键词
DIRIGENT and O-METHYLTRANSFERASE genes; Jasmonates; Rice; Salicylic acid; Stem-regulated promoters; Sugarcane
资金
- Texas Grain and Grass Gene Initiative [06-0001]
- Rio Grande Valley Sugar Growers Cooperative and Texas AgriLife Research, Texas AM System
Transcription profiling analysis identified Saccharum hybrid DIRIGENT (SHDIR16) and IY-METHYLTRANSFERASE (SHOMT), putative defense and fiber biosynthesis-related genes that are highly expressed in the stem of sugarcane, a major sucrose accumulator and biomass producer. Promoters (Pro) of these genes were isolated and fused to the beta-glucuronidase (GUS) reporter gene. Transient and stable transgene expression analyses showed that both Pro (DIR16) :GUS and Pro (OMT) :GUS retain the expression characteristics of their respective endogenous genes in sugarcane and function in orthologous monocot species, including rice, maize and sorghum. Furthermore, both promoters conferred stem-regulated expression, which was further enhanced in the stem and induced in the leaf and root by salicylic acid, jasmonic acid and methyl jasmonate, key regulators of biotic and abiotic stresses. Pro (DIR16) and Pro (OMT) will enable functional gene analysis in monocots, and will facilitate engineering monocots for improved carbon metabolism, enhanced stress tolerance and bioenergy production.
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