期刊
PLANT SCIENCE
卷 225, 期 -, 页码 102-106出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2014.05.018
关键词
Pollen germination; Pollen maturation; Sucrose phosphate synthase; Oryza sativa L
资金
- JSPS KAKENHI Grant [25450032]
- Grants-in-Aid for Scientific Research [12J06490, 24580030, 25450032, 14J10964] Funding Source: KAKEN
The molecular function of an isoform of sucrose phosphate synthase (SPS) in rice, OsSPS1, was investigated using gene-disruption mutant lines generated by retrotransposon insertion. The progeny of the heterozygote of disrupted OsSPS1 (SPS1(+/-)) segregated into SPSV+/+, SPSV1(+/-), and SPS1(-/-) at a ratio of 1:1:0. This distorted segregation ratio, together with the expression of OsSPS1 in the developing pollen revealed by quantitative RT-PCR analysis and promoter-beta-glucuronidase (GUS) fusion assay, suggested that the disruption of OsSPS1 results in sterile pollen. This hypothesis was reinforced by reciprocal crosses of SPS1(+/-) plants with wild-type plants in which the disrupted OsSPS1 was not paternally transmitted to the progeny. While the pollen grains of SPS+/- plants normally accumulated starch during their development, pollen germination on the artificial media was reduced to half of that observed in the wild-type control. Overall, our data suggests that sucrose synthesis via OsSPS1 is essential in pollen germination in rice. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据