期刊
PLANT SCIENCE
卷 215, 期 -, 页码 59-68出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2013.10.013
关键词
Capsicum; Fruit pungency; Capsaicinoids; Transcription factors
资金
- NM Agricultural Experiment Station
- USDA CSREES NIFA grant [2010-34604-20886]
- National Institutes of Health [NIGMS GM61222, S06 GM08136]
- NIFA [580657, 2010-34604-20886] Funding Source: Federal RePORTER
Accumulation of capsaicinoids in the placental tissue of ripening chile (Capsicum spp.) fruit follows the coordinated expression of multiple biosynthetic enzymes producing the substrates for capsaicin synthase. Transcription factors are likely agents to regulate expression of these biosynthetic genes. Placental RNAs from habanero fruit (Capsicum chinense) were screened for expression of candidate transcription factors; with two candidate genes identified, both in the ERF family of transcription factors. Characterization of these transcription factors, Erf and Jerf, in nine chile cultivars with distinct capsaicinoid contents demonstrated a correlation of expression with pungency. Amino acid variants were observed in both ERF and JERF from different chile cultivars; none of these changes involved the DNA binding domains. Little to no transcription of Erf was detected in non-pungent Capsium annuum or C. chinense mutants. This correlation was characterized at an individual fruit level in a set of jalapeno (C. annuum) lines again with distinct and variable capsaicinoid contents. Both Erf and Jerf are expressed early in fruit development, 16-20 days post-anthesis, at times prior to the accumulation of capsaicinoids in the placental tissues. These data support the hypothesis that these two members of the complex ERF family participate in regulation of the pungency phenotype in chile. (C) 2013 Published by Elsevier Ireland Ltd.
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