期刊
PLANT SCIENCE
卷 227, 期 -, 页码 145-156出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2014.08.001
关键词
Tomato (Solanum lycopersicum); WRKY proteins; SlDRW1; Botrytis cinerea; oxidative stress; defense response
资金
- National Basic Research Program of China [2009CB119005]
- National Key Technology R & D Program of China [2011BAD12B04]
- National High-Tech R D Program [2012AA101504]
- Research Fund for the Doctoral Program of Higher Education of China [20120101110070]
WRKY proteins comprise a large family of transcription factors that play important roles in plant responses to biotic and abiotic stresses; however, only a few of tomato WRKYs have been studied for their biological functions. In the present study, we identified a Botrytis cinerea-responsive WRKY gene SlDRW1 (Solanum lycopersicum defense-related WRKY1) from tomato. SlDRW1 is a nucleus localized protein with transactivation activity in yeast. Expression of SlDRW1 was significantly induced by B. cinerea, leading to 10-13 folds of increase than that in the mock-inoculated plants but not by Pseudomonas syringae pv. tomato (Pst) DC3000. Silencing of SlDRW1 resulted in increased severity of disease caused by B. cinerea, but did not affect the phenotype of disease caused by Pst DC3000. In addition, silencing of SlDRW1 also resulted in decreased tolerance against oxidative stress but did not affect drought stress tolerance. Furthermore, silencing of SIDRW1 attenuated defense response such as expression of defense-related genes after infection by B. cinerea. Our results demonstrate that SlDRW1 is a positive regulator of defense response in tomato against B. cinerea and oxidative stress. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
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