期刊
PLANT SCIENCE
卷 185, 期 -, 页码 259-273出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2011.11.012
关键词
Rice; Inferior spikelet; Grain-filling; ABA; Proteome; Phosphoproteome
资金
- National Natural Science Foundation of China [30871494]
- Doctoral Disciplines Foundation of Education Ministry, China [200803890006]
- Provincial Natural Science Foundation of Fujian, China [2009J01060]
- Department of Education Science and Technology Project of Fujian, China [JK2011014]
Cultivars of rice (Oryza sativa L), especially the large-spikelet-type, often fail to achieve the high yield potential due to poor grain-filling of their inferior (late-flowering) spikelets. The superior (early-flowering) spikelets normally contain more abscisic acid (ABA) than the inferior spikelets. It was speculated that ABA might play a pivotal role in the grain-filling of inferior spikelets. To understand the molecular regulation involved in this process, we employed the 2-D gel-based comparative proteomic and phosphoproteomic analyses to search for differentially expressed proteins in the inferior spikelets under exogenous ABA treatment. A total of 111 significantly differential proteins and 31 phosphoproteins were found in the inferior spikelets after treatment. Among them, 100 proteins and 23 phosphoproteins were identified by using MALDI-TOF/TOF MS. In addition, the gene expression patterns of the inferior spikelets were confirmed with RT-PCR. These differentially expressed proteins are active in defense response, carbohydrate, protein, amino acid, energy and secondary metabolisms, as well as cell development and photosynthesis. The results suggest that the grain-filling of rice inferior spikelets is regulated by ABA through some proteins and phosphoproteins participating in carbon, nitrogen and energy metabolisms. (c) 2011 Elsevier Ireland Ltd. All rights reserved.
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