期刊
PLANT SCIENCE
卷 187, 期 -, 页码 105-112出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2012.02.003
关键词
Cytokinins; Vernalization; Winter rape; Flowering
资金
- Ministry of Education, Youth and Sports of the Czech Republic [MSM 6198959216]
- Grant Agency of the Academy of Sciences CR [KAN 200380801]
- EU [CZ.1.05/2.1.00/01.0007]
The putative role of cytokinins in processes leading to reproductive development of plants was investigated by analysing the shoot apical parts of a winter cultivar of oilseed rape (Brassica napus L var. oleifera, cv. Gorczanski). The endogenous cytokinin levels were measured by liquid chromatography-tandem mass spectrometry (LC-MS) in the shoot apices of vegetative plants (grown at 20/17 degrees C with a 16/8h day/night regime) and vernalized plants (56 days at 5/2 degrees C with a 16/8 h photoperiod) at different times during floral transition. During vernalization, the content of all isoprenoid cytokinins increased significantly, coinciding well with the onset of the early stages of reproductive development. Cytokinin levels reached their maxima when most of the plants became irreversibly reproductive (after 42 days of cold treatment). cis-Zeatin riboside (unequivocally identified by quadrupole-time-of-flight MS) accounted for ca. 87-89% of the total isoprenoid cytokinin content in control and vernalized plants, whilst N-6-isopentenyladenosine (similar to 6% in control and similar to 8% in vernalized plants) and cis-zeatin (similar to 2% in control and similar to 1% in vernalized plants) were the next most abundant cytokinins. In the post-vernalization period, endogenous cytokinin levels decreased, but remained significantly higher in the reproductive plants than in the vegetative controls. These results suggest that cytokinins, especially those of the cis-zeatin type, are involved in vernalization-induced reproductive development of B. napus. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据