期刊
PLANT SCIENCE
卷 183, 期 -, 页码 14-19出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2011.10.021
关键词
Dioscorin; Dioscorea japonica; Bacterial expression; Purification; Enzymatic activity
资金
- Japan Society for the Promotion of Science
- Grants-in-Aid for Scientific Research [23228003] Funding Source: KAKEN
Dioscorin, the major tuber storage protein of yam, has been shown to possess carbonic anhydrase, trypsin inhibitor, dehydroascorbate reductase, and monodehydroascorbate reductase activities. In the present study, dioscorin from Dioscorea japonica was confirmed as a glycoprotein using the enhanced concanavalin A-peroxidase staining method, and the protein was shown to have both N- and O-glycans. Following the gene cloning, four full-length isoforms of dioscorin were expressed in Escherichia coli and purified by affinity purification and anion-exchange chromatography for structural and biochemical experiments. It was clearly observed that the recombinant dioscorins had carbonic anhydrase, trypsin inhibitor, dehydroascorbate reductase, and monodehydroascorbate reductase activities. However, the dehydroascorbate reductase and monodehydroascorbate reductase activities were markedly decreased in recombinant dioscorins compared with native dioscorin. The decreased activities were closely related to the loss of the glycosylation from the protein. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
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