期刊
PLANT SCIENCE
卷 178, 期 3, 页码 289-298出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2009.12.008
关键词
Asparagine; Asparagine synthetase gene; Boron deficiency; Boron signaling; Carbohydrates; Nicotiana tabacum
资金
- Ministerio de Educacion y Ciencia [BFU2006-05304, BFU2009-08397]
- Junta de Andalucia, Spain [BIO 266]
To date, one asparagine synthetase (AS) mRNA has been identified in Nicotiana tabacum Our results point out that this gene encodes an actual AS protein This proposal is supported by the following facts (i) Blast analysis indicates that tobacco AS gene shows increased percent identity with several AS genes of different plant species. (ii) Leaf and root AS mRNA levels were inversely correlated with soluble carbohydrate concentrations, both in the presence of light and in darkness, and the addition of exogenous sucrose repressed the root AS gene expression (iii) In light, a positive correlation between AS transcript levels and ammonium content was observed in both leaves and roots (iv) A direct correlation between leaf AS gene expression and asparagine (Asn) concentration has been found. Furthermore, quantitative real-tune PCR analyses showed that this gene expression was increased under boron (B) deficiency in roots, but not in leaves Interestingly, soluble B concentration in roots of plants subjected to B deficiency was significantly lower than that of control plants However, in leaves, there was no significant difference in soluble B content between both B treatments. We discuss the possible role of soluble B deficiency in the regulation of AS gene expression in tobacco roots. (C) 2010 Elsevier Ireland Ltd All rights reserved.
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