期刊
PLANT SCIENCE
卷 177, 期 3, 页码 222-226出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2009.04.006
关键词
RNAi; pds gene; Photosynthesis pigment; Chloroplast
资金
- [06YFGZNC01700]
- [2006GI32A100013]
- [04260]
Nicotiana tabacum plants that are silenced in pds gene have been constructed by RNAi technology. Transgenics showed different degrees of albinism and PSII efficiency was decreased significantly compared to wild type plants. The total carotenoid contents decreased. The ratio of chlorophyll (Chl) a to b was unchanged while the content of both declined. The grana stacking of thylakoid in variegation part was as normal as in the control and etioplast occurred in the white sector of leaf. The concentration of thylakoid membrane protein of molecular weight about 20 kDa was significantly increased compared to wild type plants according to the same contents of Chl. These observations suggest the exact matching of pigments and proteins is important for energy absorption and transfer in photosynthesis and mismatching of them could cause malfunction of PSII. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
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