期刊
PLANT SCIENCE
卷 176, 期 3, 页码 390-396出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2008.12.005
关键词
Grass; Lolium temulentum; Quantitative PCR; Abiotic stress
Lolium temulentum L (Darnel ryegrass) is a valuable model grass species for the study of stress in forage and turf grasses. Gene expression analysis by quantitative real-time RT-PCR relies on the use of proper internal standards. The aim of this study was to identify and evaluate reference genes for use in real-time quantitative RT-PCR for abiotic stress studies in L temulentum. Partial sequences of nine L temulentum housekeeping genes were obtained by RT-PCR using degenerate primers designed from the corresponding genes in closely related species. Primers for quantitative RT-PCR were designed based on these partial sequences. The housekeeping genes were evaluated for their expression stability in samples from plants subjected to different forms of abiotic stress. The analysis found that eEF-1 alpha and UBQ5 were the most stable and ACT11 was the least stable of the genes tested. Analysis by geNorm indicated that the two most stably expressed housekeeping genes (eEF-1 alpha and UBQ5) should be utilized and are sufficient for normalization of gene expression during stress-related studies in L temulentum. Published by Elsevier Ireland Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据