期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 84, 期 -, 页码 10-21出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2014.09.004
关键词
Natural production; Fluorescence transients OJIP; JIP-test; PSII reaction centers; Non-Q(B) reducing centers
资金
- China 863 Program [2011AA10A206]
- NSFC [31000834, 31272080]
- NSF of Jiangsu [BK2012764]
Tenuazonic acid (TeA), a phytotoxin produced by the fungus Alternaria alternata isolated from diseased croftonweed (Ageratina adenophora), exhibits a strong inhibition in photosystem II (PSII) activity. In vivo chlorophyll fluorescence transients of the host plant croftonweed, show that the dominant effect of TeA is not on the primary photochemical reaction but on the biochemical reaction after Q(A). The most important action site of TeA is the Q(B) site on the PSII electron-acceptor side, blocking electron transport beyond Q(A)(-) by occupying the Q(B) site in the D1 protein. However, TeA does not affect the antenna pigments, the energy transfer from antenna pigment molecules to reaction centers (RCs), and the oxygen-evolving complex (OEC) at the donor side of PSII. TeA severely inactivated PSII RCs. The fraction of non-Q(A) reducing centers and non-Q(B) reducing centers show a time- and concentration-dependent linear increase. Conversely, the amount of active Q(A) or Q(B) reducing centers declined sharply in a linear way. The fraction of non-Q(B) reducing centers calculated from data of fluorescence transients is close to the number of PSII RCs with their Q(B) site filled by TeA. An increase of the step-J level (V-J) in the OJIP fluorescence transients attributed to Q(A)(-) accumulation due to TeA bound to the Q(B) site is a typical characteristic response of the plants leaf with respect to TeA penetration. (C) 2014 Elsevier Masson SAS. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据