期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 83, 期 -, 页码 250-257出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2014.08.011
关键词
Colonization; HPLC; Nutritional source; Organic acids; Split-root hydroponic system
资金
- Innovative Research Team Development Plan of the Ministry of Education of China [IRT1256]
- Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions
- 111 project [B12009]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Chinese Ministry of Science and Technology [2011BAD11B03]
- Chinese Ministry of Agriculture [201103004]
The ability to colonize on plant roots is recognized as one of the most important characteristics of the beneficial fungi Trichoderma spp. The aim of this study is to prove that the utilization of organic acids is a major trait of Trichoderma harzianum T-E5 for colonization of cucumber roots. A series experiments in split-root hydroponic system and in vitro were designed to demonstrate the association between the utilization of organic acids and T-E5 colonization on cucumber roots. In the split-root hydroponic system, inoculation with T-E5 (T) significantly increased the biomass of cucumber plants compared with CM (non-inoculation with T-E5). The T-E5 hyphae densely covering the cucumber root surface were observed by scanning electron microscopy (SEM). Three organic acids (oxalic acid, malic acid and citric acid) were identified from both the CM and T treatments by HPLC and LC/ESI-MS procedures. The amounts of oxalic acid and malic acid in T were significantly higher than those in CM. All the organic acids exhibited different and significant stimulation effects on the mycelial growth and conidial germination of T-E5 in vitro. An additional hydroponic experiment demonstrated the positive effects of organic acids on the T-E5 colonization of cucumber roots. In conclusion, the present study revealed that certain organic acids could be used as nutritional sources for Trichoderma harzianum T-E5 to reinforce its population on cucumber roots. (C) 2014 Elsevier Masson SAS. All rights reserved.
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