期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 74, 期 -, 页码 141-155出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2013.11.002
关键词
Copper amine oxidase; Gene expression; Grape ripening; Polyamine catabolism; Polyamine oxidase; Vitis vinifera
资金
- Portuguese Foundation for Science and Technology (FCT) [SFRH/BPD/72070/2010]
- Auxiliar Researcher contract Ciencia [C2008-UL-BioFIG-5]
- FCT [PTDC/AGR-GPL/100919/2008]
- COST Action [FA0605 (STSM-FA0605-131210-003763)]
- BioFig Center [PEst-OE/BIA/UI4046/2011]
- CEAUL [PEst-OE/MAT/UI000612011]
- [MEC-BIO2011-29683]
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/72070/2010, PTDC/AGR-GPL/100919/2008] Funding Source: FCT
Grapes are economically the most important fruit worldwide. However, the complexity of biological events that lead to ripening of nonclimacteric fruits is not fully understood, particularly the role of polyamines' catabolism. The transcriptional and metabolic profilings complemented with biochemical data were studied during ripening of Trincadeira grapes submitted to guazatine treatment, a potent inhibitor of polyamine oxidase activity. The mRNA expression profiles of one time point (EL 38) corresponding to harvest stage was compared between mock and guazatine treatments using Affymetrix GrapeGen (R) genome array. A total of 2113 probesets (1880 unigenes) were differentially expressed between these samples. Quantitative RT-PCR validated microarrays results being carried out for EL 35 (veraison berries), EL 36 (ripe berries) and EL 38 (harvest stage berries). Metabolic profiling using HPLC and H-1 NMR spectroscopy showed increase of putrescine, proline, threonine and 1-O-ethyl- beta -glucoside in guazatine treated samples. Genes involved in amino acid, carbohydrate and water transport were down-regulated in guazatine treated samples suggesting that the strong dehydrated phenotype obtained in guazatine treated samples may be due to impaired transport mechanisms. Genes involved in terpenes' metabolism were differentially expressed between guazatine and mock treated samples. Altogether, results support an important role of polyamine catabolism in grape ripening namely in cell expansion and aroma development. (C) 2013 Elsevier Masson SAS. All rights reserved.
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