期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 64, 期 -, 页码 33-40出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2012.12.010
关键词
Cytokinins; Barley; Yield; CKX activity; IPT activity; Developmental stages
资金
- Ontario Ministry of Agriculture, Food and Rural Affairs (OMAFRA)
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Ontario Graduate Scholarship Program (OGS)
The aim of the present study was to determine if relationships between cytokinin (CK) profiles and corresponding enzymatic regulation were consistent with differences in kernel yield among commercial barley (Hordeum vulgare L) cultivars, differing in parameters relating to productivity and grown under agronomically-relevant field conditions. Quantification of the CKs at six distinct stages of kernel development by high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) revealed a significant increase in CKs, especially trans-zeatin (tZ), during the stage when grain filling occurs, with a greater CK concentration observed in the cultivar with the greatest number of kernels per spike, which was also the highest yielding cultivar. High levels of the cis-zeatin (cZ) CK form were observed at the first developmental stage, indicating a possible role in early embryo development and viability. Cytokinin oxidase (CKX; EC 1.5.99.12) activity was evaluated at each stage through a colorimetric assay, as the enzyme provides a primary mechanism for the irreversible degradation and, thus, regulation of CKs. However, no peaks in CKX activity were observed and no differences were detected at the particular stages examined. Isopentenyl transferase (IPT) gene expression was also examined and suggests that biosynthesis contributes to regulation of CK concentrations in developing barley kernels. (C) 2013 Elsevier Masson SAS. All rights reserved.
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