期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 72, 期 -, 页码 54-61出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2013.02.017
关键词
Enoyl-ACP reductase; Malus x domestica; Phloridzin; RNAi; Transient assay
资金
- New Zealand Foundation for Research, Science and Technology [C06X0812]
- New Zealand Ministry of Business, Innovation & Employment (MBIE) [C06X0812] Funding Source: New Zealand Ministry of Business, Innovation & Employment (MBIE)
Phloridzin is the predominant polyphenol in apple (Malus x domestica Borkh.) where it accumulates to high concentrations in many tissues including the leaves, bark, roots and fruit. Despite its relative abundance in apple the biosynthesis of phloridzin and other related dihydrochalcones remains only partially understood. The key unidentified enzyme in phloridzin biosynthesis is a putative carbon double bond reductase which is thought to act on p-coumaroyl-CoA to produce the dihydro-p-coumaroyl-CoA precursor. A functional screen of six apple enoyl reductase-like (ENRL) genes was carried out using transient infiltration into tobacco and gene silencing by RNA interference (RNAi) in order to determine carbon double bond reductase activity and contribution to foliar phloridzin concentrations. The ENRL-3 gene caused a significant increase in phloridzin concentration when infiltrated into tobacco leaves whilst a second protein ENRL-5, with over 98% amino acid sequence similarity to ENRL-3, showed p-coumaroyl-CoA reductase activity in enzyme assays. Finally, an RNAi study showed that reducing the transcript levels of ENRL-3 in transgenic 'Royal Gala' led to a 66% decrease in the concentration of dihydrochalcones in the leaves in the one available silenced line. Overall these results suggest that ENRL-3, and its close homolog ENRL-5, may contribute to the biosynthesis of phloridzin in apple. (C) 2013 Elsevier Masson SAS. All rights reserved.
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