期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 67, 期 -, 页码 7-14出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2013.02.016
关键词
Proteomics; Salt stress; Cucumber; Spermidine
资金
- National Basic Research Program of China (973 Program) [2009CB119000]
- National Natural Science Foundation of China [30900995, 31071831, 31272209]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- China Earmarked Fund for Modern Agro-industry Technology Research System [CARS-25-C-03]
- Ministry of Education [20100097110001]
To investigate the effects of exogenous Spd on proteomic changes under normal condition and NaCl stress of 3 days in cucumber seedling leaves, a 2-DE gel electrophoresis and MALDI-TOF/TOF MS was performed. A total of 63 differentially expressed proteins responded to salt stress or exogenous Spd treatments, and they were all successfully identified by MALDI-TOF/TOF MS. Many changes were observed in the levels of proteins involved in energy and metabolic pathways, protein metabolic, stress defense, and other functional proteins. Increased salt tolerance by exogenous Spd would contribute to higher expressions of proteins involved in the SAMs metabolism, protein biosynthesis, and defense mechanisms on antioxidant and detoxification. Meanwhile, the regulation of Calvin cycle, protein folding assembly and the inhibition of protein proteolysis by Spd might play important roles in salt tolerance. This study provides insight that may facilitate a better understanding of the salt resistance by Spd in cucumber seedlings. (C) 2013 Elsevier Masson SAS. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据