期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 71, 期 -, 页码 164-172出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2013.07.009
关键词
LSD1; PCD; Wheat; Stripe rust; Agrobacterium-mediated transient expression; Virus-induced gene silencing (VIGS)
资金
- National Basic Research Program of China [2013CB127700]
- Program for New Century Excellent Talents in University [NCET-12-0471]
- National Natural Science Foundation of China [31171795]
- 111 Project from the Ministry of Education of China [B07049]
- National High-Tech R&D Program of China [2012AA101504]
- Fundamental Research Funds for the Central Universities of China [YQ2013001]
Genetic characterization of the Arabidopsis lesion simulating disease 1 (Isd1) mutant, a lesion mimic mutant (LMM), has revealed the essential role of AtLSD1 in the negative regulation of cell death and disease resistance. The three zinc-finger motifs found in AtI5D1 revealed a novel plant-specific gene family, whose members are significantly related to programmed cell death (PCD). In this study, we characterized a functional homologue to AtLSD1, TaLSD1, in the wheat-stripe rust fungus pathosystem. The expression of TaLSD1 was differentially induced during incompatible and compatible interactions between wheat and Puccinia striiformis f. sp. tritici (Pat) and was up-regulated by oxidative stress generated by methyl viologen (MV). TaLSD1 was found to be predominately localized in the nucleus of onion epidermal cell. Transient overexpression assays in Nicotiana benthamiana demonstrated that TaLSD1 partially inhibited programmed cell death triggered by a mouse Bax protein, whereas expression of TaLSD1 alone had no influence on the phenotype of tobacco. Knocking down the expression of TaLSD1 through virus-induced gene silencing (VIGS) increased wheat resistance against Pst accompanied by an enhanced hypersensitive response (HR), an increase in PR1 gene expression and a reduction in Pst hyphal growth. Our results suggest that TaLSD1 functions negatively in regulating the plant hypersensitive cell death and is involved in disease resistance of wheat against the stripe rust pathogen. (C) 2013 Elsevier Masson SAS. All rights reserved.
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